|
|
|
| The Study on Morphologic Alteration of Fetal Mice and the Change of MeCP2 in Fetal Brain Induced by Ionizing Radiation |
| CHEN Feng1, ZHANG Feng-xiang1, TU Yu2 |
| The Erdos Central Hospital, Erdos 017000 China |
|
|
|
|
Abstract Objective In order to investigate the effect and the possible mechanism of γ-rays on neurodevelopment of fetal brain tissue as bystander effect organ. Methods pregnant kunming mice were randomly divided into blank control group, 0.5 Gy whole-body exposed group, 0.5 Gy head exposed group, 1.0 Gy whole-body exposed group, 1.0 Gy head exposed group, 2.0 Gy whole-body exposed group and 2.0 Gy head exposed group. The exposed mice were exposed with a vertical single acute dose using 60Co therapy apparatus on the 9 th day of pregnancy, and cesarean operation were performed to gain fetal mice on the 18th day of pregnancy. The number, the size, stillbirth, birth defects and abortion, and get fetal brains from live births were observed. Western-blot assay was used to detect the expression of MeCP2 protein. Results Compared with the blank control group, the rates of stillbirth, birth defects and abortion ascended as the increase of doses; the expression of MeCP2 were upregulated except 0.5 Gy whole-body exposed group, there were no significant differences between groups. Condusion When the pregnant mice were exposed to ionizing radiation in the first trimester, bystander effect in fetal brain tissue was induced, within a certain range,the incidence of deterministic effects and stochastic effects ascended as the increase of doses.
|
|
Received: 07 April 2011
|
|
|
|
|
|
|
|
|
