|
|
|
| Influence of irradiation on the expression of MMP-2/9 and VEGF in IL-4 stimulated RAW264.7 cells |
| LI Zhen, LI Hailiang, MENG Bin, SHANG Jie |
| Institute of Radiation Medicine, Shandong Academy of Medical Sciences, Jinan 250062 China |
|
|
|
|
Abstract Objective To study the effects of ionizing radiation on the secretion of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) by mouse macrophage RAW264.7 cells, providing a basis for the study of prognostic factors of tumors.Methods The experiment was divided into two parts. In the first part, IL-4 was stimulated for 12 h after being irradiated by 0 Gy, 2 Gy, 5 Gy and 10 Gy X-rays for 24 h. The second part, RAW264.7 cells were irradiated by 0 Gy, 2 Gy, 5 Gy and 10 Gy X-rays for 3 h after being stimulated by IL-4.The expression of MMP-2, MMP-9 and VEGF proteins in RAW264.7 cells were detected by Western blot.Results MMP-2, MMP-9 and VEGF expression increased with the increase of the dose in irradiation + IL-4 group (P<0.05), there was a dose-response relationship, and there was a strong correlation between MMP-2 and VEGF (P<0.001), as well asMMP-9 and VEGF (P<0.001).In the IL-4 + irradiation group, the expression levels of MMP-2, MMP-9 and VEGF increased with the increase of the dose (P<0.05), there was a dose-response relationship, and there was a strong correlation between MMP-2 and VEGF (P<0.001), as well as MMP-9 and VEGF (P<0.001).Compared with the irradiation + IL-4 group, the increase of MMP-2, MMP-9 and VEGF in the IL-4 + irradiation group was more significant (P<0.05).Conclusion Ionizing radiation can increase expression of MMP-2/9 and VEGF in mouse macrophage system RAW264.7 cells. Different irradiation methods had different effects on the expression of MMP-2/9 and VEGF in RAW264.7 cells, and this study provided a target for the treatment of tumors.
|
|
Received: 15 May 2018
|
|
|
|
|
|
[1] 张庆勇,朱武飞,王群兴.肿瘤相关巨噬细胞与肿瘤的发生发展[J].细胞与分子免疫学杂志,2013,29(1):106-108.
[2] Zhang J,Yan Y,Yang Y,et al.High infiltration of tumor-associated macrophages influences poor prognosis in human gastric cancer patients, associates with the phenomenon of EMT[J].Medicine(Baltimore),2016,95(6):e2636.
[3] Vasiljeva O, Papazoglou A, Kruger A, et al. Tumor cell-derived and macrophage-derived cathepsin B promotes progression and lung metastasis of mammary cancer[J].Cancer Res, 2006,66(10):5242-5250.
[4] Gocheva V, Wang H W, Gadea B B, et al. IL-4 induces cathepsin protease activity in tumor-associated Macrophages to promote cancer growth and invasion[J].Genes Dev, 2010,24(3):241-255.
[5] Comito G, Giannoni E, Segura CP, et al. Cancer-associated fibroblasts and M2-polarized macrophages synergize during prostate carcinoma progression[J].Oncogene, 2014,33:2423-2431.
[6] Wang R, Zhang J, Chen S, et al. Tumor-associated macrophages provide a suitable microenvironment for non-small lung cancer invasion and progression[J].Lung Cancer,2011,74(2):188-196.
[7] Cui Y L, Li H K,Zhou H Y, et al.Correlations of tumor-associated macrophage subtypes with liver metastases of colorectal cancer[J]. Asian Pac J Cancer Prev, 2013,14(2):1003-1007.
[8] Li D, Wang X, Wu J L, et al. Tumor-produced versican V1 enhances hCAP18/LL-37 expression in macrophages through activation of TLR2 and vitamin D3 signaling to promote ovarian cancer progression in vitro[J].PLos One,2013,8(2):e56616.
[9] Tugal D,Liao X,Jain MK.Transcriptional control of macrophage polarization[J].Arterioscler Thromb Vasc Biol,2013,33(6):1135-1144.
[10] Lawrence T,Natoli G.Transcriptional regulation of macrophage polarization:enabling diversity with identity[J]. Nat Rev Immunol,2011,11(11):750-761.
[11] 宛硕,黄艳,陈庭金,等.巨噬细胞极化的研究进展[J].中国病原生物学杂志,2015,10(11):1051-1054.
[12] 解海荣,李贞,李海亮,等.不同照射方式对IL-4诱导的小鼠RAW264.7细胞极化的影响[J].中国辐射卫生,2017,26(6):637-641.
[13] Mc Cawley LJ, Matrisian LM. Matrix metalloproteinases:they're not just any more[J].Curr Opin Cell Biol,2001,13(5):534-540.
[14] 姜友,刘戈,葛尔树.HER2、MMP2、Leptin在结直肠癌组织中的表达及临床意义[J].安徽医药, 2012,16(4):479-482.
[15] 蒋晓东,戴鹏.HIF1α、VEGF、VEGFR2在非小细胞肺癌组织中的表达及临床意义[J].临床肺科杂志,2011,16(3):386-388.
[16] Hovinga KE, Stalpers LJ, Van BC, et al. Radiation enhanced vascular endothelial growth factor(VEGF) secretion glioblasto mamulfiforme cell lines a clue to radioresistance[J].Neuro Oncol, 2005,74(2):99-103.
[17] 周勇,王科,何忠时,等.辐射对巨噬细胞系RAW264.7基质金属蛋白酶-9表达的影响,实用癌症杂志,2013,28(1):20-23.
[18] Riedel F, Gotte K, Schwalb J, et al.Expression of 92KD a type Ⅳ collagenase correlates with angiogenic marker 8 and poor surviving in head and neck squamous cell carcinoma[J].Int J Oncol,2000,17(6):1099-1105.
[19] Che G, Chen J,Liu L, et al. Transfection of nm23-H1 increased expression of beta Catenin, E-Cadherin and TIMP-1 and decreased the expression of MMP2,CD44v6 and VEGF and inhibited the metastatic potential of human non-small cell lung cancer line L9981[J].Neoplasma, 2006,53(6):530-537. |
|
|
|
