|
|
|
| Effects of small dose accumulative γ-irradiation on the small molecular metabolites in rats urine |
| ZHANG Huifang1,2, REN Yue2, WANG Hongli2, HU bo2, ZHANG Zhongxin2, WANG Chao2, GUO Yuefeng2 |
1. China Institute of Atomic Energy, Beijing 102413 China;
2. China Institute for Radiation Protection |
|
|
|
|
Abstract Objective To observe the effects of accumulative Cs-137(137Cs) γ irradiation at small dose on small molecular metabolites in rats urine.Methods 40 specific pathogens free grade healthy male SD rats were divided into control group and irradiated group with 20 rats in each group. The irradiated group was irradiated by 137Cs γ-ray at absorbed dose rate of 0.353 mGy/min. The accumulated dose of every day was 0.083 3 mGy. The control group was not irradiated. Urine of irradiated group was collected at first day before irradiation and 3 rd, 6 th, 9 th, 12 th day after radiation commence. Urine of control group was collected at 6th day after radiation commence. The accumulated doses were 0.00, 0.25, 0.50, 0.75, 1.00 Gy consequently. Urine of control group and irradiated group were collected at the same time. Techniques of 1H-NMR combined with principal components analysis (PCA) and partial least square discriminate analysis(PLS-DA) were used to compare the metabolite disparity of different groups.Results The results demonstrated obviously differences in urine metabolites after irradiation. There were five kind common different metabolites at different dose. They were carbohydrate, citruline, taurine, citric acid and uracil accordingly. The relative contents of five metabolites at different dose group were higher than those of the control group(P<0.05). The relative contents of taurine has the positive proportion with accumulated dose, the dose-effect relationship was ŷ=0.004-0.015x+0.058x2(R20.8821).Conclusion There are some changes in small molecular metabolites about rats urine before and after small dose accumulative γ-irradiation.
|
|
Received: 16 June 2018
|
|
|
|
|
|
[1] TYBURSKI J B, PATTERSON A D, KRISTOPHER W K, et al. Radiation metabolomics. 1. identification of minimally invasive urine biomarkers for gamma-radiation exposure in mice[J]. Radiat Res,2008,170(1):1-14.
[2] TYBURSKI J B, PATTERSON A D, KRISTOPHER K W, et al. Radiation metabolomics.2. dose and time dependent urinary excretion of deaminated purines and pyrimidines after sublethal gamma-radiation exposure in mice[J]. Radiat Res,2009,172(1):42-57.
[3] LANZ C, PATTERSON A D, SLAVIK J, et al. Radiation metabolomics.3. biomaker discovery in the urine of gamma-irradiated rats using a simplified metabolomics protocol of gas chromatography-mass spectrometry combined with random forests machine learning algorithm[J]. Radiat Res, 2009,172(2):198-212.
[4] JOHNSON C H, PATTERSON A D, KRAUSZ K W, et al. Radiation metabolomics.4.UPLC-ESI-QTOFMS based metabolomics for urinary biomarker discovery in gamma-irradiated rats[J]. Radiat Res,2011,175(4):473-484.
[5] 张慧芳,杨彪,郭月凤,等.60Co急性全身均匀照射后大鼠尿液代谢成分特征的初步研究[J].辐射研究与辐射工艺学报,2011,29(6):354-358.
[6] 张慧芳,杨彪,党旭红,等.60Co γ射线累积照射对大鼠尿液中小分子代谢物的影响[J].中华放射医学与防护杂志,2012,32(4):358-361.
[7] WILLIAMS R E, LENZ E M, LOWDEN J S, et al. The metabonomics of ageing and development in the rat:an investigation into the effect of age on the profile of endogenous metabolites in the urine of male rats using 1H NMR and HPLC-TOF MS[J]. Mol Biosyst,2005,1(2):166-175.
[8] GOUDARZI M, WEBER W, MAK T D, et al. Development of urinary biomarkers for internal exposure by cesium-137 using a metabolomics approach in mice[J]. Radiat Res,2014,181(1):54-64.
[9] WATERS N J, WATERFIELD C J, FARRANT R D, et al. Metabonomic deconvolution of embedded toxicity:application to thioacetamide hepato-and nephrotoxicity[J]. Chem Res Toxicol,2005,18(4):639-654.
[10] SOUIDI M, SCANFF P, GRISON S, et al. Effects of ionizing radiation on the activity of the major hepatic enzymes implicated in bile acid biosynthesis in the rat[J]. C R Biol,2007,33(12):861-870.
[11] WU G. Synthesis of citrulline and arginine from proline in enterocytes of postnatal pigs[J].Am J Physiol,1997,272(6Pt 1):G1382-G1390.
[12] CRENN P, HANACHI M, NEVEUX N, et al. Circulating citrulline levels:a biomarker for intestinal functionality assessment[J].Ann Biol Clin (Paris),2011,69(5):513-521.
[13] ZEZULOV M, BARTOU KOV M, HL DKOV E, et al. Citrulline as a biomarker of gastrointestinal toxicity in patients with rectal carcinoma treated with chemoradiation[J]. Clin Chem Lab Med,2016,54(2):305-314.
[14] GRIMALDI D, GUIVARCH E, NEVEUX N, et al. Markers of intestinal injury are associated with endotoxemia in successfully resuscitated patients[J]. Resuscitation,2013,84(1):60-65.
[15] PAN L, WANG X, LI W, et al. The intestinal fatty acid binding protein diagnosing gut dysfunction in acute pancreatitis:a pilot study[J].Pancreas,2010,39(5):633-638.
[16] WOO H K, KIM E K, JUNG Y H, et al. Reduced early dried blood spot citrulline levels in preterm infants with meconium obstruction of prematurity[J].Early Hum Dev,2015,91(12):777-781.
[17] CRENN P, DE TRUCHIS P, NEVEUX N, et al. Plasma citrulline is a biomarker of enterocyte mass and an indicator of parenteral nutrition in HIV-infected patients[J].Am J Clin Nutr,2009,90(3):587-594.
[18] CRENN P, NEVEUX N, CHEVRET S, et al. Plasma l-citrulline concentrations and its relationship with inflammation at the onset of septic shock:a pilot study[J].J Crit Care,2014,29(2):1-6.
[19] GOUDARZI M,CHAUTHE S, STRAWN S J, et al. Quantitative metabolomic analysis of urinary citrulline and calcitroic acid in mice after exposure to various types of ionizing radiation[J]. Int J Mol Sci,2016,17(5):782-799. |
|
|
|
