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| Left-half-body-irradiation Induced Mouse Bone Marrow Hematopoietic Cells DNA Damage in Non-irradiation Area |
| YANG Long1, WANG Yi-ping2, RONG Shu1, et al |
| Institute of Military Medical Research Center of Nanjing Military District Nanjing 210002 China |
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Abstract Objective:To investigate the DNA damage of mouse bone marrow hematopoietic cells in non-irradiation area after being irradiated by way of left-half-body.Methods 6~8 weeks male Kunming strain mice were randomly divided into 4 groups i.e.normal control (NC), total-body-irradiated (TBI), left-half-body-irradiated (LHBI), and total-body-shield-irradiated (TBSI).Half-body-irradiated model were made with two pieces of 5cm×8cm×16cm overlapped lead bricks shielding right-side body and irradiated with 8.0 Gy 60Coγ-ray.The TNF-α, SOD, MDA in mouse serum were measured and the DNA damages of bone marrow hematopoietic cells were observed by comet assay and the frequency of polychromatic erythrocytes micronucleated (fMPCE).Results In the left-half-body-irradiated condition, The TNF-α and MDA were increased and the SOD was decreased in serum remarkably (compared with NC, P<0.01);In non-irradiation area, the fMPCE and the percentage of bone marrow hematopoietic cells with comet-like tail, were aggravated significantly.Conclusions Our study suggest that the local irradiation result in the DNA damage of bone marrow hematopoietic cells in non-irradiation area, and the increasing of TNF-αand reactive oxygen or free radicals may play an important role in the damages.
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Received: 07 July 2006
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