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| Effect of tea polyphenols on chromosomal aberration of CHL cells and micronucleus formation induced by radiation |
| LI Qihui1, WANG Fang1, WANG Xiude1, ZHANG Huibin1, GU Qiamin1, SHANGGUAN Tao2, ZOU Zhongmin2, TANG Mutao1 |
1. Jinan Military Center of Disease Prevention and Control, Jinan 250014;
2. Institute of Toxicology, College of Preventive Medicine, Third Military Medical University |
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Abstract Objective To study the effect of tea polyphenols(TP) on chromosomal aberration of Chinese hamster fibroblast cells(CHL) and micronucleus formation induced by radiation.Methods CHL cells (with and without S9)were divided into control group, positive control group, radiation group and TP protective group which were given TP12.5、25、50 μg/ml. Both cells were exposed to 2 Gy 60Co γ rays except control group and positive control group. After cultured in 1640 for 24 hours, cells were harvested for the preparation of chromosome. Subsequently, the ratio and the type of chromosomal aberration were examined. 50 mice were divided into control group, 60Co γ ray radiation group and TP protective group which were given a gavage of TP 725, 145, 29 mg/kg. 10 mice each group. After 14 days the mice were received 6 Gy 60Co γ radiation and were executed after radiation exposure 24 h. By means of bone marrow smear and Giemsa staining, the number of micronucleus cells per 1 000 polychromatic erythrocyte (PCE) was counted.Results The ratios of chromosomal aberration of TP protective group(25、50 μg/ml) were significantly decreased compared with radiation group. S9 has no effect on the type and rate of chromosomal aberration. Compared with radiation group, the micronucleus ratio of TP protective group (725 mg/kg and 145 mg/kg) were significantly decreased(P<0.05).Conclusion TP could partly resist chromosomal aberration of CHL cells and micronucleus formation induced by radiation. TP has protective effects on damage of chromosome.
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Received: 11 June 2017
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