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| Effect of low-dose X ray on human umbilical vein endothelial cells (HUVECs) secreting von Willebrand factor (vWF) |
| WANG Yifan1, ZHENG Yingying2, XIE Qi2, LIU Ju2 |
1. Weifang Medical University, Weifang 261053 China;
2. Medical Research Center, Shandong Provincial Qianfoshan Hospital |
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Abstract Objective To determine the effects of low-dose (0.1 Gy) X-ray on secretion of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVECs).Methods HUVECs were irradiated at the doses of 0 Gy (control) and 0.1 Gy (200 cGy/min) by a medical linear accelerator. The proliferation, viability and apoptosis of the irradiated HUVECs were examined through MTT assay, Trypan Blue exclusion and flow cytometry. The secretion of vWF was detected through ELISA and Western blot of the culture media of the irradiated HUVECs.Results There were no statistically significant differences between the control group and the experimental group in proliferation, viability and apoptosis of HUVECs. However, the secretion of vWF in the experimental group was increased significantly.Conclusion Low-dose (0.1 Gy) X-ray irradiation does not change the proliferation, viability and apoptosis of HUVECs, but increases the secretion of vWF from HUVECs.
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Received: 01 December 2017
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