|
|
|
| Effect of MgSO4 on the Expression of C-fos Gene and the Abilities of Learning and Memory in the Rat Brain with Radiation Induced Injuries |
| ZHANG Wei1, WANG Li-li2, ZHOU Ju-ying2, et al |
| Shandong Academy of Occupational Health Occupational Medicine, Jinan 250062 China |
|
|
|
|
Abstract Objective To investigate the effect of magnesium sulfate(MgSO4) on the nerves function after hemisphere irradiation and explore possible mechanism of the effect.Methods Mature Sprague-Dawley rats were randomly divided into 3 groups:blank control group(n=26),experimental control group(n=32) and experimental group(n=32).To selecte rats randomly from the experimental control group and the experimental therapy group for 1/2 brain irradiation to a single-fraction maximal dose of 20Gy using 5MeV electrons.Magnesium sulfate was injected intraperitoneally into the rats that from the experimental group before and after irradiation for total seven times.The expression of c-fos gene was observed in the hippocampal formation of the rat brain with the immunofluorescence technique in 24h after irradiation.The learning and memory results in Y-maze test were detected 8 weeks after irradiation respectively with the other rats and then had the pathomorphology observation.Results Compared with the blank control group,the number of c-fos immunopositive cells in the hippocampal formation of experimental control group were increased markedly(P<0.01),and the learning and memory results of Y-maze test in the irradiation control group declined significantly(P<0.01).Compared with the irradiation control group,the experimental group's Fos immunopositive cells decreased significantly(P<0.05),and also the cognitive function of learning attempt times(at 4 and 8 weeks,P<0.01) and memory reappearance times(P<0.05) were significantly different from that in the irradiation control group.Conclusion Magnesium sulfate can promote the recovery of the rats' cognitive impairment in the early stage after irradiation,and may play a role of protecting hippocampus neurons by the way of decreasing the expression of Fos protin in hippocampal formation.
|
|
Received: 14 April 2009
|
|
|
|
|
|
|
|
|
