|
|
|
| Pathological Change of Esophageal Carcinoma Cells of Tumor-bearing Nude Mice Combined Retinoblastoma 94 Gene with Radiation |
| YANG Wen-yan, WANG Yan, XU Chang, DU Li-qing, SUN Zhi-juan, LI Jin, LIU Qiang, WANG Qin |
| Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192, China |
|
|
|
|
Abstract Objective To reveal pathological change of carcinoma cells of tumor-bearing nude mice combined retinoblastoma Rb94 gene with radiation. Methods The model of tumor-bearing nude mice was built by inoculating K150 cells. The cohorts were divided into five groups:blank control、Ad-LacZ、Ad-Rb94、radiation and Ad-Rb94 combined with radiation (combining group for short). The pathological change of esophageal carcinoma was detected. Results Tumors of control group and Ad-LacZ group grew actively. Meanwhile, treatment with Ad-RB94 and/or radiation resulted in cancer cell growth inhibition and apoptosis. Compared with other ones, tumors of combination treatment group had fewer karyokinesis and lower level of nuclei hyperchromasia. Conclusion Rb94 gene combined with ionizing radiation shows synergism effect on the inhibition of tumor growth and increases apparently the curative effect on esophageal carcinoma cells.
|
|
Received: 14 August 2016
|
|
|
|
|
|
|
|
|
