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| Primary Quantitative Analysis of the mtDNA4977bp Deletion Induced by Ionizing Radiation in Human Peripheral Blood Using real-time PCR |
| DUAN Zhi-kai, LIU Jian-gong, GUO Wan-long, et al |
| China Institute for Radiation Protection, Taiyuan 030006 China |
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Abstract Objective To observe the influence of mtDNA4977bp deletion induced by different dose of γ ray in human peripheral blood in order to explore the feasibility of mtDNA4977bp deletion as biodosimeter.Methods Human peripheral blood samples were collected from three healthy donors and irradiated by γ ray,MtDNA4977bp deletion was detected by real-time PCR.Results It indicated that that from the range of 0~8 Gy,the relationship between mtDNA4977bp deletion and irradiation dose represents certain curvilinear correlation (Y=1.2693+1.0660X+0.0198X2).Conclusion We find that γ ray has influence on the mtDNA4977bp deletion,so it may be an important biodosmeter in future.
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Received: 10 December 2010
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