|
|
|
| Effect of γ-ray Irradiation With Different Dosage on the Expression of BMPR2 in Human Hepatocytes |
| LI Jian-guo, YIN Jing-jing, YUAN Hui, QIN Xiu-jun, ZHANG Wei, WEN Jian-hua |
| Department of Radiation Medicine and Environment Medicine, China Institute for Radiation Protecton, Taiyuan 030006 China |
|
|
|
|
Abstract Objective To investigate the effect of irradiation of different dosage and different time on the expression of bone morphogenetic protein receptor 2 (BMPR2) in Human Hepatocytes, and provide evidence for early damage of hepatocyte. Methods The human hepatocytes were irradiated with 60Co γ-ray at 0.1, 0.2, 0.5, 1, 2 and 4 Gy, BMPR2 expression was detected by real-time fluorescent quantitative PCR after irradiation 6, 12 and 24h. Results Melting curve of β-actin gene and BMPR2 with different dosage and time was the single peak, which means the specific ampliations. Relative quantitative ratio of different dosage irradiated 6 h lower than 1 showed down-regulated. Ratio of irradiated 12 h greater than 1 showed up-regulated. Irradiation 24 h, 0.2 and 2.0 Gy showed down-regulated, and 0.1, 0.5, 1 and 4 Gy showed up-regulated. Conclusion BMPR2 does not show dose-effect relationship irradiated by different dosage, but shows obviously differential expression with different time.
|
|
Received: 06 July 2012
|
|
|
|
|
|
|
|
|
