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| Study on the influence factors of automatic analysis of dicentric chromosomes for biodosimetry |
| FENG Junchao1, ZHANG Lin2, DAI Hong1, LIU Yulong1, BIAN Huahui1, CHEN Weibo1, WANG Youyou1, MA Ya2, LIU Wei2 |
1. The Second Affiliated Hospital of Soochow University, Suzhou 215004 China;
2. Institute of Radiation Medicine, Shandong Academy of Medical Sciences |
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Abstract Objective To study the influences of different experimental conditions on the results of automatic analysis of dicentric chromosomes for biodosimetry. Methods The dose effect curve of "automatic analysis of dicentric chromosomes" fitted by our laboratory to estimate the dose of samples prepared in different conditions after the genetic workstation automatically analyzed the dicentric chromesomes. Results The results of biodosimetry are not affected by the conditions of 50 hours at 20℃ of blood placement before inoculation, different cell suspensions concentration, different degree of dispersion in the metaphases, Wright-Giemsa staining depth, repeated scanning times, sensitivity parameter of genetic workstation and distribution positions of the metaphases on the slide (P>0.05 and have no significant difference). Conclusion Automatic analysis of dicentric chromosomes is not sensitive to the general influence factors in the experiments, and is conducive to the promotion among different laboratories.
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Received: 30 March 2018
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