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| Preparation technique for the study of chromosome, micronucleus, and transformation rate of peripheral blood lymphocytes |
| LING Pan, HE Ling, WANG Xiaofeng, ZHAO Qinhong, ZHAO Yi, CHEN Xiyang |
| Sichuan Center for Diseases Control and Prevention, Chengdu 610041 China |
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Abstract Objective To explore the preparation technique for improving the quality and efficiency of lymphocyte examination. Methods The peripheral venous blood of a person without radiation exposure was used as the control group, and the blood from the same person irradiated with 60Co γ-ray at a dose rate of 1.002 Gy/min and a dose of 2.8 Gy was used as the experimental group. The lymphocytes were cultured at 37℃ for 48 h, added with colchicine, and cultured for another 20 h. The culture medium was divided into hypotonic and non-hypotonic groups and pre-fixed separately. Then the two groups of culture medium were mixed, purified, sectioned, stained, and observed. The same specimen was used for experiment with the standard method. Results According to the results of improved method and standard method, the chromosome aberration rates of the experimental group were 75.8% and 74.0%, respectively, and those of the control group were 0; the micronucleus cell rates of the experimental group were 7.8‰ and 7.2‰, respectively, and those of the control group were 1.2‰ and 1.4‰, respectively; the lymphocyte transformation rates of the experimental group were 43.6% and 43.2%, respectively, and those of the control group were 65.4% and 66.2%, respectively. Conclusion There are no significant differences between the improved and normal methods in terms of chromosome aberration rate, micronucleus cell rate, and lymphocyte transformation rate of peripheral blood lymphocytes. The improved method can shorten the preparation time, reduce reagent use, and improve the quality of section. Observation can be performed on one slide, which shortens the overall observation time and improves the reporting efficiency.
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Received: 24 May 2021
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