X射线对小胶质细胞M1和M2型极化的影响

doi:10.13491/j.issn.1004-714X.2021.03.001

2021, Vol. 30

Issue (3): 247-252 DOI: 10.13491/j.issn.1004-714X.2021.03.001

Radiobiology/Original Articles

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The effect of X-ray on microglial M1 and M2 polarization

HUANG Rongrong^1,2, ZHOU Yan², GUO Ling², LIU Liyuan², XUE Yizhe², LAI Panpan², JING Yuntao², XU Hui³, ZHANG Qinfeng¹, DING Guirong²

1. School of Public Health, Shandong First Medical University, Tai’an 271000 China;
2. Department of Radiation Protection Medicine, Department of Military Preventive Medicine, Air Force Medical University, Xi’an 710032 China;
3. College of Radiology, Shandong First Medical University, Tai’an 271000 China

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Abstract Objective To investigate the effect of X-ray on the polarization of mouse microglia BV-2 cells.Methods BV-2 cells at the logarithmic growth stage were randomly divided into the Sham irradiation group and 10 Gy irradiation group. The latter group was given a single X-ray irradiation at a dose of 1.28 Gy/min for 7 min 49 s. The activation rate of BV-2 cells was observed and analyzed under a microscope at 1, 3, 6, 24 h and 48 h after irradiation.The changes of cell morphology were observed by HE staining and immunofluorescence staining; The levels of M1-type activation markers (TNF-α and IL-1β) and M2-type activation marker TGF-β1 in the supernatant of BV-2 cells were detected by ELISA. The levels of polarization-related proteins of M1-type (CD86 and iNOS) and M2-type (CD206) in BV-2 cells were detected by Western blotting.Results Morphological results showed that BV-2 cells became larger, and their protrude became coarse and shorter, showing "amoeba" like changes after 10 Gy X-ray irradiation. Compared with the Sham group, the activation rate of BV-2 cells was significantly increased at 3 h, and reached the peak at 6 h, and began to recover at 48 h after irradiation. ELISA results showed an obvious increase in the level of TNF-α and TGF-β1 48 h after irradiation.The level of IL-1β showed a transient decrease at 3～6 h, increased at 24 h, and reached the peak 48 h after irradiation. Western blotting results showed that CD86 protein level did not change significantly at each time points after irradiation, and iNOS protein level increased significantly at 1, 6, 24 h and 48 h after irradiation. A fluctuating change in CD206 protein level was found after irradiation.Conclusion 10 Gy X-ray irradiation can induce the activation of BV-2 cells in vitro, and the polarization type changes with the time after irradiation.

Key words： Ionizing Radiation X-ray Microglia Cells M1 Polarization M2 Polarization

Received: 21 December 2020

PACS:

Q691

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	Articles by authors
	HUANG Rongrong
	ZHOU Yan
	GUO Ling
	LIU Liyuan
	XUE Yizhe
	LAI Panpan
	JING Yuntao
	XU Hui
	ZHANG Qinfeng
	DING Guirong

Cite this article:

HUANG Rongrong,ZHOU Yan,GUO Ling, et al. The effect of X-ray on microglial M1 and M2 polarization[J]. , 2021, 30(3): 247-252.

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http://www.zgfsws.com/EN/10.13491/j.issn.1004-714X.2021.03.001 OR http://www.zgfsws.com/EN/Y2021/V30/I3/247

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