|
|
|
| Effects of the expression of miRNA in human peripheral blood after different doses of gamma rays |
| DONG Juancong, YUAN Yayi, DANG Xuhong, WANG Jingjie, MENG Qianian, WANG Chao, LIU Hongyan, REN Yue, ZHANG Zhongxin, ZUO Yahui |
| China Institute of Radiation Protection, Taiyuan 030006 China |
|
|
|
|
Abstract Objective In order to provide early diagnostic indicators for radiation damage, high throughput miRNA sequencing and bioinformatics technology were used to screen radiation-sensitive miRNA in human peripheral blood.Methods The peripheral blood of healthy adult males was given 0.2 Gy and 2.0 Gy gamma rays, and total RNA was extracted at 6 hours after irradiation. Differential miRNA was obtained by high-throughput miRNA sequencing technology, and partially differential miRNA was verified by qRT-PCR. Target genes of common differential miRNA were predicted by mirdbV6 and Target Scan7.1 database, and bioinformatics analysis was analyzed using KEGG. Results The number of differential miRNAs in human peripheral blood was different with at 6 h after different doses radiation. There were 10 differential miRNAs in 0.2 Gy group, and 2 were up-regulated, and 8 were down-regulated. In 2.0 Gy group, there were 9 up-regulated and 12 down-regulated miRNAs. And the differences of both two groups were statistically significant (P < 0.05). There were 5 miRNAs showed significant changes in both groups. RT-PCR results showed that there were 4 miRNAs, including mir-23c, mir-1287-5p, mir-219a-3p and mir-320d, which were consistent with the sequencing results. Bioinformatics results showed that common differential miRNAs may affect cell proliferation, cell apoptosis, DNA damage repair and immune regulation by regulating the MARK, RAS, P53, RIG-I and other signaling pathways.Conclusion The mir-23c, mir-1287-5p, mir-219a-3p and mir-320d are expected to become new blood makers of radiation injury.
|
|
Received: 15 March 2020
|
|
|
|
|
|
[1] 吕玉民. 染色体畸变在急、慢性辐射损伤评估中的意义专家解析[J]. 中国辐射卫生,2019,28(4):349-354
[2] Sproull M T, Camphausen K A, Koblentz G D. Biodosimetry: a future tool for medical management of radiological emergencies[J]. Health Secur, 2017, 15(6): 599-610
[3] Li S, Lu X, Feng J B, et al. Identification and validation of candidate radiation-responsive genes for human biodosimetr[J]. Biomed Environ Sci, 2017, 30(11): 834-840
[4] 李刚强, 濮亚斌, 王寅. 小剂量辐射致小鼠血液中microRNA表达改变[J]. 实用预防医学,2016,23(4):405-408
[5] Bugden M, Billing S, Mak K C, et al. Ionizing radiation affects miRNA composition in both young and old mice[J]. Int J Radiat Biol, 2019, 95(10): 1404-1413
[6] Małachowska B, Tomasik B, Stawiski K, et al. Circulating microRNAs as biomarkers of radiation exposure: a systematic review and meta-analysis[J]. Int J Radiat Oncol Biol Phys, 2020, 106(2): 390-402
[7] 袁龙, 付熙明, 雷翠萍, 等. 全国核辐射突发事件卫生应急能力现状分析[J]. 中国辐射卫生,2019,28(1):28-32
[8] 林仲武, 王琪, 王治东. 辐射生物剂量计的研究现状及发展方向[J]. 中华放射医学与防护杂志,2017,37(10):799-802
[9] Jacobs A R, Guyon T, Headley V, et al. Role of a high throughput biodosimetry test in treatment prioritization after a nuclear incident[J]. Int J Radiat Biol, 2020, 96(1): 57-66
[10] Anindo M I, Yaqinuddin A. Insights into the potential use of microRNAs as biomarker in cancer[J]. Int J Surg, 2012, 10(9): 443-449
[11] 李刚强, 朱瑞, 周海亚. 60Coγ辐射致小鼠血液中microRNA表达改变及意义[J]. 河南预防医学杂志,2016,27(6):401-405
[12] Aryankalayil M J, Chopra S, Makinde A, et al. Microarray analysis of miRNA expression profiles following whole body irradiation in a mouse model[J]. Biomarkers, 2018, 23(7): 689-703
[13] 李刚强, 巢胜吾, 潘瑞林, 等. X射线辐射损伤对介入工作人员血液中miRNA表达的影响[J]. 中国煤炭工业医学杂志,2017,20(11):1301-1304
[14] 王津晗, 路倩颖, 纪凯华, 等. 基于准路阻侧学的放射性肠损伤基因动态变化[J]. 中华放射医学与防护杂志,2018,3(2):81-85
|
| [1] |
LIU Hanxiao, DENG Daping, LI Jieqing, FANG Lianying, MAO Xuesong, LI Weiguo, MA Ya, HOU Dianjun, ZHU Wei, QIAO Jianwei, JIA Ximing. Analysis of some blood indexes of interventional radiation workers[J]. , 2020, 29(3): 211-214. |
|
|
|
|
