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| Ionizing radiation induced miRNA-150 changes in peripheral blood |
| HOU Dianjun, MA Ya, LI Jieqing, MAO Xuesong, ZHU Wei |
| Institute of Radiation Medicine Shandong Academy of Medical Sciences Shandong First Medical University, Jinan 250062 China |
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Abstract Objective To study the differential regulations of miRNA-150 in human peripheral blood after ionizing radiation.Methods Venous blood was collected and exposed to 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy X-ray and set a control group at the same time. Blood samples were inoculated in culture bottles containing 10% calf serum and cultured in 37℃ incubators after radiation.miRNA-150 changes were detected after 8 and 24 hours post radiation by PCR. Sequence alignment for miRNA150 use tbools software. Predict the target gene of miRNA150 use Starbase datebase.Results After 8 h with 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy radiation,miRNA-150 expression levels were 0.66、0.68、0.74、0.75、0.63, compared with the unirradiated group, the results were statistically significant; miRNA-150 expression levels were 0.94、0.95、0.71、0.59、0.67 after 24 h, the expression was downregulated compared to 8 h, compared with the unirradiated group, the results were statistically significant. Human and mouse have the same sequence in miRNA-150-5p. There is a single base difference in the Seed sequence region of miRNA-150-3p. The target gene prediction shows that there is a highest probability of BASP1,MAP3K12 and ZBTB4 as the target genes of miRNA-150 for human.Conclusion The expression of miRNA-150 in peripheral blood after radiation is related to radiation injury and has a potential serum marker for radiation injury.
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Received: 18 June 2019
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