不同剂量电离辐射对人外周血淋巴细胞DNA损伤修复相关基因转录水平的影响

doi:10.13491/j.issn.1004-714X.2020.01.002

2020, Vol. 29

Issue (1): 7-12 DOI: 10.13491/j.issn.1004-714X.2020.01.002

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Effects of different dose ionizing radiation on the transcriptional level of DNA damage repair related genes in human peripheral blood lymphocytes

CHEN Huifeng¹, TAN Siwen^1,2, YAN Xuehua¹, WANG Qia¹, YANG Yuhua¹, ZOU Jianming¹

1. Guangdong Province Hospital for Occupational Disease Prevention and Treatmen, Guangdong Provincial Key Laboratory of Occupational Diseases Prevention and Treatment, Guangzhou 510300 China;
2. Guangdong Medical University

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Abstract Objective To investigate the effects of different dose ionizing radiation on the transcriptional level of DNA damage and repair related genes (hOGG1, MGMT, PPP2R1A and PPP2R2D) in human peripheral blood lymphocytes.Methods 30 mL peripheral blood was extracted from a volunteer and was subpackaged into seven heparin lithium-anticoagulant tubes. Human peripheral blood was irradiated by using ⁶⁰Co-γ rays (doses of 0, 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2 Gy) at a dose rate of 0.38 Gy/min. Peripheral blood lymphocytes were isolated after 12 h and then total RNA was extracted for quantitative and purity analysis. Real-time fluorescence quantitative polymerase chain reaction method was used to analyze the changes of transcriptional level of hOGG1, MGMT, PPP2R1A and PPP2R2D gene.Results The transcription levels of hOGG1, MGMT and PPP2R2D gene all reached a peak of 0.1 Gy. The transcription level of PPP2R1A reached a peak of 0.2 Gy. Compared with the control group, the transcription levels of hOGG1 gene increased significantly at 0.1 Gy and 0.2 Gy(P < 0.05). The transcription levels of MGMT gene at 0.1 Gy, 0.2 Gy and 0.8 Gy were all significantly higher than that in the control group (P < 0.05). Compared with the control group, the transcription levels of PPP2R1A gene were increased significantly at 0.2 Gy (P < 0.05). Compared with the control group, the transcription level of PPP2R1A gene was decreased significantly at 3.2 Gy (P < 0.05). Compared with the control group, the transcription levels of PPP2R2D gene increased significantly at 0.1 Gy 0.2 Gy, 0.4 Gy and 0.8 Gy. Compared with the control group, the transcription level of PPP2R2D gene was decreased significantly at 3.2 Gy (P < 0.05).Conclusion Different dose ionizing radiation could induce changes in the transcriptional levels of the genes of DNA damage repair enzyme including, hOGG1, MGMT, PPP2R1A and PPP2R2D. Suitable low dose ionizing radiation (0.1~0.2 Gy) may induce radiation hormesis or adaptive response of cells.

Key words： Low Dose Ionizing Radiation DNA Damage Repair hOGG1 Gene MGMT Gene PPP2R1A Gene PPP2R2D Gene

Received: 19 July 2019

PACS:

Q691

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	CHEN Huifeng
	TAN Siwen
	YAN Xuehua
	WANG Qia
	YANG Yuhua
	ZOU Jianming

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CHEN Huifeng,TAN Siwen,YAN Xuehua, et al. Effects of different dose ionizing radiation on the transcriptional level of DNA damage repair related genes in human peripheral blood lymphocytes[J]. , 2020, 29(1): 7-12.

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http://www.zgfsws.com/EN/10.13491/j.issn.1004-714X.2020.01.002 OR http://www.zgfsws.com/EN/Y2020/V29/I1/7

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