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| Influence of X-ray on the P53 Gene in Human Peripheral Blood Lymphocytes |
| JIN Wen-wei1, CAI Ting2 |
| The Second People's Hospital of Hangzhou City, Hangzhou 310016, China |
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Abstract Objective To evaluate the reliability and safety of varying X-ray dosage.Methods Peripheral lymphocytes of five healthy volunteers were processed by varying X-rays, then detect the P53 gene mutation in 5~9 exons by PCR-SSCP silver staining, investigate the 249 th codon's mutation by PCR-RFLP, through immunohistochemistry staining monitor the abnormal expression of P53 and screen the apoptosis employing the Bio-dUTP terminal labeling technology incluced by DNA terminal transferase.Results The frequency of apoptosis represents transparent dose-dependent manner with X-ray.When exposed to X-ray>50 cGy after 48 h, the apoptosis group has evident difference compared with the control (P<0.01), while 20 cGy group exhibits prominent difference after 72 h (P<0.01), but no difference in 5~10 cGy (P>0.05).After treating peripheral lymphocytes with 5~200 cGy X-ray and culturing 96 h, utilizing PCR-SSCP to determine the mutation in 5~9 exons, there was no single strand DNA abnormal migration.PCR-RFLP result indicates no mutation in the hotspot site-249 codon, and there was no obviously abnormal expression of P53 in immunohistochemistry staining.Conclusion The apoptosis of peripheral lymphocytes is sensitive to the X-ray, and this can be a guidline or model reflecting the body state when exposing to the radiation.
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Received: 16 January 2002
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