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| The Preparation of Recombinant Interleukin-6 and Its Protection for Irradiated Guinea-pigs |
| WANG Jun-fu1, ZHANG Jie1, ZHANG Jian-hua1, et al |
| Shandong Academy of Medical Sciences, Jinan 250062, China |
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Abstract Objective To purify recombinant human interleukine-6 from gene-engineered E.coli and study the effects of IL-6 on survival period and platelet count of irradiated guinea-pigs.Methods Recombinant human IL-6 was expressed in gene-engineered E.coli and purified with reversed phase chromatography.Purified IL-6 was then administered to irradiated guinea-pigs.Results IL-6 could significantly prolong the survival period and increase the platelet count of irradiated guinea-pigs.Conclusion IL-6 showed its effective protection for irradiated guinea-pigs.
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Received: 24 January 2002
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