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| Assessment of DNA Damage in Radiation Staff Using Comet Assay |
| LI Rui, WANG Yan, DU Li-qing, XU Chang, FAN Sai-jun, LIU Qiang |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine. Tianjin 300192 China |
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Abstract Objective To observe the DNA damage level of radiation workers in different work types and work time. Methods DNA single strand break were detected by using comet assay, and all the comet were analysed by the CASP software. TDNA%, TL, TM and OTM were detected in the present study. Statistics analysis was performed using SPSS13.0 software. Results TDNA%,TL,TM and OTM in the radiation group were higher than those of control group significantly(F=5.26, P < 0.01) and the significant difference was found in the different types of work or different lengths of service (F=2.67, P < 0.01). Conclusion Obvious correlation was found among different types of work or different lengths of service.
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Received: 06 March 2014
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