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| Radiosensitization Effect of Valproic Acid on Human Hepatocellular Cell Line SMMC-7221 |
| CHEN Cheng, LIU Kai, JI Bo, WANG Ying-chao, LIU Ya-hui |
| Hepatopancreatobiliary Department The No.1 Hospital of Jilin University, Changchun 130021 China |
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Abstract Objective To investigate radiosensitization effect of valproic acid (VPA) on human hepatocellular cell line SMMC-7221 and its mechanism. Methods MTT was used to detect the proliferation and IC50 of SMMC-7221 cell. FCM was adopted to analyze the changes in cell cycle and apoptosis level. Results After co-cultured with VPA, the IC50 of SMMC-7221 cell was 41.26、9.04、2.87mmol/L. VPA + IR could significantly inhibit the cell proliferation, increase apoptosis level, cause G0/G1 phase arrest and decrease the decreased the percentages of S phrase. Conclusion VPA could enhance the cell-killing effect of SMMC-7221 cell as a radio-sensitizer, which was associated with apoptosis and cell cycle arrest
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Received: 26 November 2013
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