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Investigation on Accordance of DNA Double-strand Break of Blood Between in Vivo and in Vitro Irradiation Using Single Cell Gel Electrophoresis |
LIU Qiang, JIANG En-hai, LI Jin, et al |
Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical Collage, Tianjin 300192 China |
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Abstract Objective To observe the consistency of DNA double-strand break between in vivo and in vitro irradiation, as a prophase study in radiation biodosimetry using single cell gel electrophoresis (SCGE).Methods Detect DNA double-strand break after whole-body and in vitro radiation in mice lymphocytes using neutral single cell gel electrophoresis.The comet images were processed by CASP software and all the data were analysed by SPSS12.0.Results There is no difference between in vivo and in vitro irradiation group in HDNA%、TDNA%、CL、TL、TM and OTM.Conclusion The result of neutral single cell gel electrophoresis shortly after in vitro irradiation can precisely reflect the DNA double-strand break of lymphocytes in whole-body irradiation.
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Received: 19 November 2005
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