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| Study of the DHEA Protective Effect on DNA Damage Induced by Radiation in Mice |
| ZHANG Li-juan, MA Rui, WANG Yue-ying, LIU Qiang, LU Lu, ZHANG Jun-ling, MENG Ai-min, LI De-guan |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key laboratory of Molecular Nuclear Medicine, Tianjin 300192 China |
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Abstract Objective To investigate the protective effects of the DHEA (17 a α-D-homo ethynycl dstradid-3-accetale) on DNA damage in mice lead by radiation. Methods IRM-2 inbred mice injected with DHEA consecutively three times 3d、2d、1d before irradiation. Neutral single cell gel electrophoresis was used, and DNA double strand breaks of the mouse lymphocytes were detected after 2 Gy gamma ray irradiation. Results The index illustrated by comet tail DNA content (TDNA%), comet tail length(TL),tail moment(TM) and Olive tail moment (OTM) in DHEA groups with low, middle and high doses, ethinyl estradiol (EE2) group and Neal estradiol (523) group were significantly lower than those of the control group. There was statistically significant difference compared with the control group (P < 0.01). The index demonstrated by TDNA, TL, TM and OTM in the DHEA middle dose group was significantly lower than that of EE2 and the 523 groups. And there were significant differences compared with the 523 and EE2 groups (P < 0.01). Conclusion DHEA can significantly reduce the lymphocyte DNA double strand breaks and has obvious protective effect on mice DNA damage caused by radiation.
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Received: 07 May 2015
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