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| Study on Biological Effect on Mice and Use Safety of 830nm Semiconductor Laser |
| LI Ke-qiu, LI Jian, MIAO Xu-hong, LIU Shu-juan, LI Guang |
| Department of Biology, Tianjin Medical University, Tianjin 300070 China |
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Abstract Objective To study biological effect on mice by 830nm semiconductor laser in different dosage, and determine the optimal irradiating dosage by observing and analyzing the immunoregulation and cytogenetical damage of mice after irradiation.Methods The spleen and thymus areas of Kunming mice were irradiated in vitro by 830 nm semiconductor laser of 30mW for 5 min, 10 min and 20 min per day respectively, then the blood samples were collected from orbital vein.Further, the spleen tissue and sternum marrow were collected soon after the mice were killed.Afterwards, IgG, dopamine, serotonin in serum were detected respectively.Besides these, the rate of lymphocyte transformation and the rate of micronuclei in marrow polychromatic erythrocytes were also determined.Results With the extending of irradiating time, the detected factors changed differently.Statistically, there were differences in IgG concentration and the rate of lymphocyte transformation between 10 min group, 20 min group and control group respectively, but no difference between each experimental group were found.Compare with control group, serotonin concentration in 10 min group increased, and there was statistical difference between these two groups, while there was no difference in dopamine concentration among each group.Besides these, the rate of micronuclei in 20 min group increased.Conclusion In this study, irradiation by semiconductor laser for appropriate time can improve immunofunction of mice, but irradiation in high dosage will result in the damage of genetic material.The optimal time of irradiation by 830 nm semiconductor laser was 10 min.
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Received: 13 March 2006
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