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| The mRNA Expression of XRCC Repair Genes in Mice after γ-Ray Radiation |
| WANG Qin, YUE Jing-yin, LI Jin, et al |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To investigate the role of XRCC repair genes in radioresistance of IRM-2 inbred mice.Methods Northern hybridization was used to measure mRNA expression of XRCC1 and XRCC5 genes in IRM-2 mice and their parental mice ICR/JCL and 615 after exposure to different doses of γ-ray radiation at different postirradiation time.Results The levels of XRCC1 and XRCC5 mRNA expression in control IRM-2 mice were higher significantly than those in their control parental mice (P<0.01 and P<0.05).The mRNA expression of XRCC genes in ICR/JCL and 615 mice all increased to some extent after exposure 1, 2 and 4Gy radiation.But the levels were significantly higher at 2h postirradiation (P<0.05).The levels of XRCC mRNA expression in IRM-2 mice did not increase significantly compared with the control mice after exposure 1 and 2Gy radiation.But the levels of XRCC1 and XRCC5 mRNA expression increased markedly at 4Gy 1h postirradiation (P<0.05 and P<0.01).Conclusion The basal levels of XRCC1 and XRCC5 mRNA expression in IRM-2 mice were high.The high level of XRCC5 mRNA expression was involved in the repair of DNA double strand breaks induced by higher dose radiation, which perhaps was one of radioresistance causes of IRM-2 mice.
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Received: 22 November 2005
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