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| Apoptosis of Bone Marrow Leukemia Cells in Mice after Low Dose Radiation at Different Time |
| Li Guang-zhou, Yu Ming-ming, Li Xian-Sep, et al |
| Department of Nuclear Medicine, Weifang Medical College Affiliated Hospital, Weifang 261031 China |
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Abstract Objective To investigate the apoptosis of bone marrow leukemia cell in mice after low dose radiation (LDR) at different time and the experimental basis for LDR auxiliary therapy on leukemia.Methods WEHI-3 cells were injected into BALB/c mice through tail veins to make an experimental mice model of myelomonocytic leukemia.60 leukemia mice models were divided half-and half.30 mice models in experimental group were irradiated with LDR of 75mGy at the same time while the others 30 in the control group were not.6 mice models with LDR and 6 mice models without LDR would be killed at the time the 1st day, the 2nd day, the 3rd day, the 5th day and the 10th day after LDR in order to extract bone marrow samples.The apoptosis percentage of leukemia cells in bone marrow was examined.Results The apoptosis percentage of leukemia cells in experimental group was increasing after LDR and went to top on the 2nd day and the 3rd day.The apoptosis percentage of leukemia cells was remarkably different between experimental and control group, all P<0.05.Conclusion LDR could significantly increase the apoptosis percentage of bone marrow leukemia cells in mice.Its mechanism is remarkably different in kill and wound of big dose radiation to tumour cells.It is probably related to of the increase immune exciting response as to promote some cytokine secretion.in leukemia mice.
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Received: 05 March 2007
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