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| The Establishment of Leucopenia Model on KM-HL Mice |
| SHEN Xiu, ZHOU Ze-wei, WANG Yin-lan, et al |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To provide theoretical foundations for KM-HL mice used as experimental mice on leucopenia model.Methods Cyclophosphamide and 137Cs source γ rays irradiated at 7.5Gy once the whole body (dose rate:0.80645Gy/min) were given on KM-HL mice to induce mice leucopenia model, samples were obtained to examine the variation of physiological indexes such as blood, hematopoietic system, immunity, heart and liver etc.Results The research shows, in normal KM-HL mice in the leucopenia experiment, some of the physiological indexes of WBC, DNA, spleen, thymus, heart shows that they are deeply injured (P<0.001), only the Cyclophospamide group's liver is lightly injured, without any statistic difference.Conclusion Either giving cyclophosphamide or 137Cs source γ rays irradiating once all over the body, the physiological indexes such as blood, hematopoietic system, immunity, heart and liver etc of KM-HL mice are descended, compared with the control group (P<0.001), therefore it can be used as experimental mice on leucopenia.
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Received: 28 January 2007
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