|
|
|
| Effects of Ionizing Radiation with Different Dose in P-gp Expression of Colorectal Carcinoma HCT-8 Cells |
| MA Lin1, LI Xiao-feng1, ZHOU Huai-li1, et al |
| Fifth Hospital of Sun Yat-Sen University, Guangdong Zhuhai 519000 China |
|
|
|
|
Abstract Objective To detect the method of reversing tumor multidrug resistance, and study the expression of colorectal carcinoma MDR1 genetic expression product P-glycoprotein (P-gp) in irradiated HCT-8 cells of with different dose ionizing radiation.Methods Flow cytometry was used to detect the change of P-gp.Results positive cells percentage of P-gp increase obviously (P<0.01) after irradiated with 2Gy.When pre-irradiated with low dose (0.05Gy, 0.1Gy), then irradiated with high dose, positive cell percentage of P-gp increase obviously (P<0.05), and so dose the positive cell percentage of P-gp (P<0.01) in the group of 0.2Gy+2Gy.Compared with the group of 2Gy, positive cell percentage of P-gp decreases obviously (P<0.05) in the group of 0.1Gy+2Gy.Couclusion low dose ionizing radiation can reverse radiogenic tumor multidrug resistance with high dose.
|
|
Received: 22 February 2008
|
|
|
|
|
|
|
|
|
