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| The Impact of the Expression of Protein HIF-1α and p53 in Hep-2 Human Laryngeal Cancer Cell Induced by 60Coγ-ray in the Condition of Reoxygenation after Hypoxiaon |
| WANG Bao-shan, LIU Yan-mei, SONG Dong-mei, et al |
| Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Hebei Medical University, Shi jia zhuang 050031 China |
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Abstract Objective To simulate the microenvironment of cancer cell in vivo, establish models of Hep-2 laryngeal cancer cell line of different oxygen supplying, and to investigate the impact of reoxygenation after hypoxia on 60Coγ-ray induced apoptosis in Hep-2 human laryngeal cancer cell line.Methods Human laryngeal cancer cell line Hep-2 was diviced into 3 groups:group A (normoxia), group B (hypoxia), group C (reoxygenation after hypoxia).HIF-1α mRNA level was determined by RT-PCR.Flow cytometry (FCM)was used to measure the protein level of HIF-1α and p53 and to detect cell apoptosis.The protein level of HIF-1α and p53 was also determined by immunohistochemistry.Results Hypoxia evidently increased the mRNA and protein level of HIF-1α in Hep-2 cell, at the same time increased the protein level of p53, after reoxygenation the mRNA and protein level of HIF-1α become lower, and dose protein level of p53.Compared with group B, the apoptosis rate of group C become higher and show a visible difference (P<0.05).Conclution Hypoxia decreased the effect of 60Coγ-ray on inducing apoptosis in Hep-2 human laryngeal cancer cell line.After reoxygenation the effect was obviously improved.But at this time, the protein level of p53 did not show high expression, indicating that the high apoptosis induced by 60Coγ-ray in the condition of reoxygenation after hypoxiaon were related to some other factor except p53.
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Received: 24 January 2008
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