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| Inhibiting of Adenovirus-Mediated IL-21 Gene on Growth of Ovarian Carcinoma Cells |
| WANG Qin, LI Jin, SONG Li, et al |
| Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To study the effect of inhibition on the growth of ovarian carcinoma cells by introduction of foreign IL-21 gene.Methods The recombinant IL-21 gene adenovirus vector was transfected into ovarian carcinoma cell line ES-2.The cells growth curve and cells cycle of ES-2 cells were detected by MTT assay and flow cytometry respectively.Results The growth of ES-2 cells transfected with Ad-IL-21 was slower than that of Ad-lacZ and blank control,especially at 3ird day after transfection(P<0.05).Compared with Ad-lacZ and blank control,cell growth at 5th day after infection was inhibited significantly(P<0.05).Cells of G1 phases transfected with Ad-IL-21 increased,cells of G2 and S phases decreased.Conclusion The recombinant IL-21 gene adenovirus vector can transfect ES-2 cells efficiently and inhibit ES-2 cell growth.
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Received: 04 May 2009
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