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| Effects of Adenovirus-mediated IL-21 Gene Vector with γ-ray Radiation on Growth of Cervical Carcinoma Cells |
| SHAO Ying1, CAO Yong-zhen1, LI Jin2, et al |
1. Department of Radiation Oncology, General Hospital of Tianjin Medical University, Tianjin 300052, China;
2. Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192 China |
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Abstract Objective To study the effect of inhibition on the growth of cervical carcinoma cells by introduction of foreign IL -21 gene vector with γ-ray. Methods The recombinant IL -21 gene adenovirus vector was transfected into cervical carcinoma cell line Hela. Hela cells were irradiated with 6Gy 137Cs γ-ray after 6 hours transfection The cells growth curve and cells cycle of Hela cells were detected by MTT assay and flow cytometry respectively. Results The growth of Hela cells transfected with Ad-IL -21, radiation and Ad-IL -21 combined with radiation group was all inhibited. The group of Ad-IL -21 combined with radiation resulted in greater inhibition of cells growth, reaching 44%, compared with Ad-IL-21 infection and radiation group. Cells of G1 phases of Hela cells for Ad-IL-21 combined with radiation group were the highest, reaching 88.9%, while those of S phases were the lowest, reaching 1%. Conclusion Ad-IL-21 gene transfer combined with radiation show synergism for the inhibition of cervical cancer Hela cells growth.
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Received: 13 July 2010
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