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| The Primary Study of the Radiosensitive Effect on the H460 Cell Line by Human Lactotransferrin High Expression In vitro |
| WANG Yong, WANG Yan, DU Li-qing, et al |
| Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Tianjin 300192 China |
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Abstract Objective To investigate the possible radiosensitive effect of human Lactotransferrin(hLF) high expression in lung cancer cell,we hereby constructed and transfected a recombinant vector pBC1 containing exogenous hLF gene.Methods Firstly the recombinant plasmid pBC1-hLF was transferred into H460 and the hLF expression was evaluated by western blotting.Then we test the cell viability,apoptosis and clone formation after radiation along with the standard hLF treatment control.Results The results showed that the hLF gene had been successfully transfect into H460 cells and hLF high expression can reduce the clone formation after radiation(P<0.01),and inhibit the cell proliferation with induced apoptosis(P<0.01).And the sensitization ratio of hLF treated and pBC1-hLF transfected were 1.29,1.59.Conclusion Our primary data shows that hLF high expression can radio sensitize the H460 cell in vitro.
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Received: 08 June 2009
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