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| The Radiosensitivity of Tamoxifen to Human Glioma Cells SHG-44 |
| NING Ping, LIU Fen-ju, JIANG Jia-gui, et al |
| School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection Jiangsu, Suzhou 215123 China |
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Abstract Objective To study the radiosensitivity of tamoxifen to the human glioma cell lines SHG-44.Methods 3H-TdR incorporation assay was used to detect DNA synthesis in glioma cells SHG-44,which were exposed to 60Co γ-irradiation with 0、1、3、5、7、9 Gy.When T lymphocyte and B lymphocyte from human peripheral blood were stimulated by phytoagglutinin(PHA) and lipopolysaccharide(LPS),the effect of tamoxifen on proliferation of T lymphocyte and B lymphocyte was assessed by the same method.Results Tamoxifen shifted the radiation dose-response curve to the left,with the decrease of D0 values.Tamoxifen(2~8μmol/L) shows no significant inhibitory effects on proliferation of T lymphocyte and B lymphocyte,and there was no significant difference between TAM group and the control group(P>0.05).Conclusion Tamoxifen could increase radiosensitivity of glioma cells SHG-44 to γ-irradiation,and it had no effect on the proliferation of T lymphocyte and B lymphocyte at the concentration of 2~8μmol/L.
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Received: 10 October 2008
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