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| Adaptive Response of DNA Damage on Thymocytes Induced by Low Doses Radiation of 131I in Mice |
| WAN Shu-li, DONGYE Guang-zhi, PANG Xin, ZHANG He, WANG Yan, ZHANG Fang-cheng, HE Qi-li, MENG Qing-yong |
| Institute of Medical Laboratory Guangdong Medical College, Dongguan 523808 China |
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Abstract Objective To study the adaptive response of DNA damage on thymocytes induced by low dose radiation of 131I in mice. Methods Doses of D1 and D2 were determinted by the methods of agarose gel electrophoresis and gray value measurement. D1 was the low dose of 131I, D2 was the high dose of 131I that could cause obvious DNA damage on thymocytes. Then the divergence of DNA damage on thymocytes was compared between D1 + D2 group and D2 group. The interval time between D1 and D2 was 12 h. Results According to the results of agarose gel electrophoresis and gray value measurement,the clarity and gray value of DNA ladder were increased with the increasing doses of 131I. D2 was 106 Bq/g. The clarity and gray value of DNA ladder were significantly decreased in D1 + D2 group compared to D2 group. Furthermore, the groups of 102 Bq/g + 106 Bq/g and 103 Bq/g + 106 Bq/g decreased most. Conclusion Low dose radiation of 131I could induce adaptive response of DNA damage on thymocytes in mice.
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Received: 28 August 2014
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