LI Shi-long, LIU Bo, Chen Wen-zhong, WU Qiu-ji, YU Tao
Objective To investigate radiation injuries induced by C-arm X ray scan in mice. Methods 72 healthy male kunming mice were randomly divided into control group, single, twice, four, eight and sixteen times of C-arm X ray scan. The mice were administered with different frequency of C-arm X ray scan; After 24 h, T-AOC,GSH and ROS were detected by T-AOC Assay Kit (ABTS method), GSH Assay Kit and ROS Assay Kit, respectively. The γ-H2AX level in peripheral lymphocytes and bone marrow cells (cell cycle phase) were analyzed by flow cytometry. Results Compared with control, four, eight and sixteen times of scan increased ROS level, but eight and sixteen times of scan decreased GSH level, sixteen times of scan significantly decreased T-AOC levelin thyroid. Compared with control, four, eight and sixteen times of scan increased γ-H2AX level in peripheral lymphocytes, eight and sixteen times of scan increased γ-H2AX level in bone marrow cells. Compared with control, sixteen times of scan increased the percentages of G0/G1 and G2/M phases, while eight and sixteen times of scan decreased the percentages of S phases. Conclusion C-arm X ray scan produced different degrees of radiation injuries in mice.