|
|
|
| Preparation of Monoclonal Antibodies and Immunoradiometric Assay Kit Against Squamous Cell Carcinoma Antigen and its Clinical Value in Diagnosis of Cancer |
| WANG Li-kai1,2, DONG Quan-wen3, PAN Xue-ji3, HUANG Li-juan2,4, ZHANG Xu4 |
| Union Chemiluminescence Diagnosis Reagent Technology(Tianjin) Ltd, Union Medical & Pharmaceutical Technology Group(Tianjin) Ltd, Key Lab of Immunoassay Technology for Tianjin's Companies, Tianjian Biological Pharmacy(Tianjin) Co, Ltd Tianjin 300457 China |
|
|
|
|
Abstract Objective To prepare monoclonal antibodies and immunoradiometric assay kit against Squamous Cell Carcinoma Antigen (SCCA) and study its clinical value in diagnosis of Cervical cancer and lung squamous cell carcinoma. Methods With immune BALB/c mice with recombinant SCCA antigen, monoclonal antibodies were generated through splenic cell and myeloma cell fusion and screening, and immunoradiometric assay kit for SCCA was developed with 2 paired Mc Antibodies. And its sensitivity and clinical value in diagnosis of Cervical cancer and lung squamous cell carcinoma were evaluated. Results 2 paired monoclonal antibodies with high affinity and high specialty were screened and immunoradiometric assay kit for SCCA was prepared. The detected sensitivity of the kit is 0.08ng/mL, and its clinical sensitivity for Cervical cancer and lung squamous cell carcinoma was 92.24% and 89.52% respectively, specificity was 98.75%. Conclusion This immunoradiometric assay kit for SCCA has high sensitivity and specificity in detection of Cervical cancer and lung squamous cell carcinoma and could be used in clinical diagnosis.
|
|
Received: 12 January 2016
|
|
|
|
|
|
|
|
|
