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| The Dose-response Relationship of T-cell Receptor Gene Mutation of Rat Peripheral Blood Lymphocytes Induced by X-ray |
| SHI Yan, LIU Wei, HOU Dian-jun, et al |
| Institute of Radiation Medicine, Shandong Academy of Medicine Sciences, Jinan 250062 China |
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Abstract Objective To investigate the does-response relationship of T-cell receptor from rat peripheral blood lymphocyte induced by X-ray.Methods Freshly isolated peripheral lymphocytes from rat were irradiated with X-ray ranged from 0 Gy to 3 Gy and cultured with phytohemagglutinin Concanavalin A and interleukin-27days, The mutant frequencies of TCR gene were detected by flow cytometry.Results TCR genes mutation increases with the dose incense in the cultured the rat lymphocytes 7 days and it exists favourable dose-response relationship.Data were found to be fitted out optimal by a quadratic polynomial dose-response modle, which could be described by regression equation:TCRMF=TCRMF=1.615+9.979D+1.712D2 (F=146.781, P<0.01, Radj2=0.867).Conclusion The favourable dose-response relationship between TCRMF induced by X ray and dose can be found from this study.
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Received: 02 December 2009
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