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| Changes of NO,NOS and AIF Gene in Mouse Testis Induced by Low Dose Radiation |
| FANG Fang1, WANG Zhicheng2, LIU Yang2, et al |
| Department of Nutrition and Food Hygiene, School of Public Health, Jilin University, Changchun 130021 China |
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Abstract Objective To study changes of NO content,NOS activity and AIF gene in mouse testis induced by low dose radiation.Methods To measure changes of NO content and NOS activity in mouse testis tissue after irradiation with 0~0.2 Gy with enzymic method.At the same time,they were measured after 0~24 h irradiation with 0.075 Gy;We also observe changes of apoptosis inducing factor (AIF)gene in mouse testis tissue after irradiation with 0~0.2 Gy with real-time PCR and Western blot,and AIF mRNA and protein expressions were meausured after 0~24 h irradiation with 0.075 Gy.Results The content of NO and activity of NOS in mouse testis tissue increased with the dose increasing after 12 h irradated with different dose X-rays,it increased with the dose raising and reached the maximum with 0.075 Gy (P<0.05),and kept high level with 0.1 and 0.2 Gy.AIF mRNA increased with dose raising,it reached the peak value when dose was 0.1 Gy;but AIF protein expression reached maximum with 0.075 Gy.The NO content and NOS activity increased with time prolongation after irradiation with 0.075 Gy,NO content reached peak value at 24 h,but NOS activity at 12 h,AIF mRNA and protein increased with time prolongation.mRNA reached maximum at 24 h,but protein at 12 h.Conclusions After irradiation with low dose radiation,NO content,NOS activity and AIF gene expression in mouse testis spermatogenic cells increased and had time and dose effect regularity.
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Received: 24 September 2009
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