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| Comparison of the Radiation-protective Effection among Different Types of Sijunzi Decoction |
| QU Gong-lin, LI Chen, SHAO Shuai, QI Xue-song, WANG Chun-yan, TONG Peng |
| Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Centre for Disease Control and Prevention, Beijing 100088 China |
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Abstract Objective To observe the radiation-protective effect of different types of Sijunzi Decoction on AHH-1 and BALB/c mice.Methods CCK-8 Assay Kit was used to detect the survival rate of AHH-1 cells treated by the extract of different types of Sijunzi Decoction for 48h on different dosages. According to the results, the radiation-protective effect of different types of Sijunzi Decoction on AHH-1 single-irradiated by 4.0 Gy 60Co γ-rays was investigated. One hundred and twenty BALB/c mice aged 6-8 weeks were divided by random number table method into normal control group, radiation group, Ginseng Sijunzi Decoction(10:9:9:6) group, Ginseng Sijunzi Decoction(2:2:2:1) group, and Codonopsis Sijunzi Decoction (2:2:2:1) group. Radiation-damaged model mice were single-irradiated by 3.5 Gy 60Co γ-rays, and the extract of different types of Sijunzi Decoction were administered intragastrically before and after the irradiation. The activity index including body weight, thymus index, spleen index, bone marrow micronucleus rate and apoptosis of spleen cells were detected.Results Compared to the radiation group, the survival rate of irradiated AHH-1 cells were increased by Codonopsis Sijunzi Decoction(2:2:2:1) at the dosage of 250μg/mL(P<0.05). The body weight recovery of mice were promoted by Ginseng Sijunzi Decoction(2:2:2:1) and Codonopsis Sijunzi Decoction(2:2:2:1) at 3d after irradiation(P<0.05). At 7d after irradiation, the mice thymus index were elevated by Ginseng Sijunzi Decoction(10:9:9:6) (P<0.05), also the increasement of mice bone marrow micronucleus rates were inhibited by Ginseng Sijunzi Decoction(10:9:9:6) (P<0.05) at 3d after irradiation. Besides, Ginseng Sijunzi Decoction(10:9:9:6) has the significant effect on reducing the apoptosis of spleen cells at 3d or 7d after irradiation. Codonopsis Sijunzi Decoction(2:2:2:1) has significant effect on inhibiting the increasement of mice bone marrow micronucleus rates and reducing the apoptosis of spleen cells at 3d or 7d after irradiation. Ginseng Sijunzi Decoction(2:2:2:1) has the same effect with Codonopsis Sijunzi Decoction(2:2:2:1) and it can also raise mice thymus index, spleen index greatly at 7d after irradiation.Conclusion On the same proportion of the principal drug, Ginseng Sijunzi Decoction was more effective. On the other hand, Ginseng Sijunzi Decoction(2:2:2:1) was more effective than Ginseng Sijunzi Decoction(10:9:9:6). The experiment results indicate the correctness of the following points of view:a. Ginseng is more appropriate for the principal drug, b. the proportion of (2:2:2:1) is more effective on the radiation-protective effection, c. Codonopsis Sijunzi Decoction and the proportion of (10:9:9:6) also play a role in the radiation-protective effection.
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Received: 09 November 2016
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