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| Study on MiR-200 Family Expression in the Stages of Transformation of Human Bronchial Epithelial Cells Induced by α-Particles |
| GAO Gang, YANG Ying-jie, LIU Jian-xiang, TIAN Mei, SU Xu |
| China CDC Key Laboratory of Radiological Protection and Nuclear Emergency, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevetion, Beijing 100088 China |
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Abstract Objective To investigate miR-200 family expression in the stages of transformation of human bronchial epithelial cells induced by α-particles. Methods Real Time PCR was used to detect miR-200 family and ZEB family and epithelial mesenchymal transition marker expression in transformed cells induced by α-particles. Results The downregulation of mature miR-200a, miR-200b, miR-200c and miR-141 expression in RHT35 cells was detected by Real Time PCR compared with R15H20 and BEP2D cells. The mRNA level of ZEB1 and ZEB2 in RHT35 cells upregulate compared with R15H20 and BEP2D cells, P < 0.01. The mRNA level of E-cadherin in RHT35 cells downregulate and N-cadherin upregulate compared with R15H20 and BEP2D cells, P < 0.01. Conclusion MiR-200 family are differentially expressed in the stages of transformation of human bronchial epithelial cells induced by α particles.
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Received: 20 November 2012
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. [J]. , 2012, 21(1): 78-80. |
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