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| BMSCs promote M2 macrophage polarization to attenuate acute radiation-induced lung injury |
| ZHANG Xinhui1,2, NIU Shiying1,3, YAO Shutong1,2, ZHANG Xiaoyue1, CAO Xuetao1,2, GAO Xue1,2, ZHAO Guoli4, CHEN Jingkun2, ZHANG Yueying1,2 |
1. Department of Pathology, the First Affiliated Hospital of Shandong First Medical University, Jinan 250013 China;
2. Department of Pathophysiology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117 China;
3. Department of Pathology, Linfen Central Hospital, Linfen 041099 China;
4. Department of Pathology, Liaocheng Infectious Disease Hospital, Liaocheng 252002 China |
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Abstract Objective To investigate the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) for radiation-induced lung injury (RILI) and the underlying mechanism. Methods Forty-five healthy adult male C57BL/6 mice were randomly divided into control, model, and BMSCs groups. The model and BMSCs groups received a single irradiation dose of 20 Gy to the chest, while the control group did not receive X-ray irradiation. For the BMSCs group, an injection of 1×106 BMSCs cells was administered via the tail vein within 6 h after irradiation. In the 5th week, the lung tissue was taken to observe pathological changes with HE staining; examine the expression of the inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) with immunohistochemical staining; observe the polarization of macrophages with immunofluorescence staining; and measure the expression of the epithelial-mesenchymal transition markers E-cadherin, N-cadherin, and vimentin proteins by Western blot. Results After radiation, the model group developed pulmonary vasodilation and congestion with septal thickening and inflammatory cell infiltration, and these changes were markedly reduced in the BMSCs group. The model group showed significantly down-regulated expression of IL-6 and TNF-α compared with significantly increased levels in the model group (P < 0.01, P < 0.05). Treatment with BMSCs significantly increased the polarization of lung macrophages towards the M2 type, while significantly decreasing the abnormally increased N-cadherin and vimentin levels in RILI mice (P < 0.05, P < 0.01). Conclusion BMSCs have therapeutic effects for RILI mice, which may be through promoting macrophage polarization from M1 to M2.
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Received: 12 September 2023
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