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| Research progress of imaging flow cytometry in automatic analysis of radiation biodosimetric indicators |
| LIU Xiaoming, CHENG Jiao, CUI Shuangshuang, DANG Xuhong, DONG Juancong |
| China Institute of Radiation Protection, Taiyuan 030006 China |
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Abstract A large number of people would be exposed to irradiation in large-scale nuclear and radiation accidents or nuclear terrorist attacks. Therefore, it is urgent to establish rapid and high-throughput biodosimetry for in triage, providing a basis for emergency management. Imaging flow cytometry (IFC) possesses the high through put advantages of traditional flow cytometry and the sensitivity and specificity of microscope, and has a good application prospect in the research and development of rapid, automated, and high-throughput biological dose estimation technology. This article reviews the application progress of IFC in biodosimetry, and provides a reference for the development of biological dose estimation and detection equipment for large-scale nuclear and radiation accidents.
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Received: 23 August 2022
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[1] Obrador E, Salvador-Palmer R, Villaescusa JI, et al. Nuclear and radiological emergencies: biological effects, countermeasures and biodosimetry[J]. Antioxidants, 2022, 11(6): 1098. DOI: 10.3390/antiox11061098
[2] 凌攀, 何玲, 王晓凤, 等. 外周血淋巴细胞染色体、微核和转化率检查制片技术探讨[J]. 中国辐射卫生,2021,30(6):677-681. DOI: 10.13491/j.issn.1004-714X.2021.06.004
Ling P, He L, Wang XF, et al. Preparation technique for the study of chromosome, micronucleus, and transformation rate of peripheral blood lymphocytes[J]. Chin J Radiol Health, 2021, 30(6): 677-681. DOI: 10.13491/j.issn.1004-714X.2021.06.004
[3] 钱小莲, 方慧莲. 核型自动扫描系统在淋巴细胞染色体畸变检测中的价值[J]. 中国辐射卫生,2020,29(5):470-472. DOI: 10.13491/j.issn.1004-714X.2020.05.006
Qian XL, Fang HL. The value of karyotype automatic scanning system in the detection of lymphocyte chromosome aberrations[J]. Chin J Radiol Health, 2020, 29(5): 470-472. DOI: 10.13491/j.issn.1004-714X.2020.05.006
[4] 吕玉民. 染色体畸变在急、慢性辐射损伤评估中的意义专家解析[J]. 中国辐射卫生,2019,28(4):349-354,360. DOI: 10.13491/j.issn.1004-714X.2019.04.001
Lv YM. Expert interpretation on the significance of chromosomal aberration in the assessment of acute and chronic radiation damage[J]. Chin J Radiol Health, 2019, 28(4): 349-354,360. DOI: 10.13491/j.issn.1004-714X.2019.04.001
[5] 方亦龙, 韩大飞, 檀学文, 等. 成像流式细胞术在检测巨噬细胞吞噬肿瘤细胞中的应用[J]. 安徽医科大学学报,2021,56(10):1670-1674. DOI: 10.19405/j.cnki.issn1000-1492.2021.10.032
Fang YL, Han DF, Tan XW, et al. Application of imaging flow cytometer in detecting tumor cell phagocytosis[J]. Acta Univ Med Anhui, 2021, 56(10): 1670-1674. DOI: 10.19405/j.cnki.issn1000-1492.2021.10.032
[6] Yang FF, Zhang FJ, Yang LP, et al. Establishment of the reference intervals of whole blood neutrophil phagocytosis by flow cytometry[J]. J Clin Lab Anal, 2021, 35(8): e23884. DOI: 10.1002/jcla.23884
[7] Wilkins RC, Rodrigues MA, Beaton-Green LA. The application of imaging flow cytometry to high-throughput biodosimetry[J]. Genome Integr, 2017, 8: 7. DOI: 10.4103/2041-9414.198912
[8] Basiji DA. Principles of Amnis imaging flow cytometry[J]. Methods Mol Biol, 2016, 1389: 13-21. DOI: 10.1007/978-1-4939-3302-0_2
[9] Doan M, Vorobjev I, Rees P, et al. Diagnostic potential of imaging flow cytometry[J]. Trends Biotechnol, 2018, 36(7): 649-652. DOI: 10.1016/j.tibtech.2017.12.008
[10] Shuryak I, Turner HC, Perrier JR, et al. A high throughput approach to reconstruct partial-body and neutron radiation exposures on an individual basis[J]. Sci Rep, 2020, 10(1): 2899. DOI: 10.1038/s41598-020-59695-9
[11] Seager AL, Shah UK, Brüsehafer K, et al. Recommendations, evaluation and validation of a semi-automated, fluorescent-based scoring protocol for micronucleus testing in human cells[J]. Mutagenesis, 2014, 29(3): 155-164. DOI: 10.1093/mutage/geu008
[12] Bryce SM, Bemis JC, Avlasevich SL, et al. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity[J]. Mutat Res, 2007, 630(1/2): 78-91. DOI: 10.1016/j.mrgentox.2007.03.002
[13] Rodrigues MA, Beaton-Green LA, Kutzner BC, et al. Automated analysis of the cytokinesis-block micronucleus assay for radiation biodosimetry using imaging flow cytometry[J]. Radiat Environ Biophys, 2014, 53(2): 273-282. DOI: 10.1007/s00411-014-0525-x
[14] Rodrigues MA, Probst CE, Zayats A, et al. The in vitro micronucleus assay using imaging flow cytometry and deep learning[J]. NPJ Syst Biol Appl, 2021, 7(1): 20. DOI: 10.1038/s41540-021-00179-5
[15] Wang Q, Rodrigues MA, Repin M, et al. Automated triage radiation biodosimetry: integrating imaging flow cytometry with high-throughput robotics to perform the cytokinesis-block micronucleus assay[J]. Radiat Res, 2019, 191(4): 342-351. DOI: 10.1667/RR15243.1
[16] International Atomic Energy Agency. Cytogenetic dosimetry: applications in preparedness for and response to radiation emergencies[R]. Vienna: IAEA, 2011.
[17] Flegal FN, Devantier Y, Marro L, et al. Validation of QuickScan dicentric chromosome analysis for high throughput radiation biological dosimetry[J]. Health Phys, 2012, 102(2): 143-153. DOI: 10.1097/HP.0b013e3182307758
[18] Beaton LA, Ferrarotto C, Kutzner BC, et al. Analysis of chromosome damage for biodosimetry using imaging flow cytometry[J]. Mutat Res, 2013, 756(1/2): 192-195. DOI: 10.1016/j.mrgentox.2013.04.002
[19] Beaton-Green LA, Rodrigues MA, Lachapelle S, et al. Foundations of identifying individual chromosomes by imaging flow cytometry with applications in radiation biodosimetry[J]. Methods, 2017, 112: 18-24. DOI: 10.1016/j.ymeth.2016.08.006
[20] Brind'Amour J, Lansdorp PM. Analysis of repetitive DNA in chromosomes by flow cytometry[J]. Nat Methods, 2011, 8(6): 484-486. DOI: 10.1038/nmeth.1601
[21] Doležel J, Kubaláková M, Cíhalíková J, et al. Chromosome analysis and sorting using flow cytometry[J]. Methods Mol Biol, 2011, 701: 221-238. DOI: 10.1007/978-1-61737-957-4_12
[22] Beaton-Green LA, Wilkins RC. Quantitation of chromosome damage by imaging flow cytometry[J]. Methods Mol Biol, 2016, 1389: 97-110. DOI: 10.1007/978-1-4939-3302-0_6
[23] Lee Y, Wang Q, Shuryak I, et al. Development of a high-throughput γ-H2AX assay based on imaging flow cytometry[J]. Radiat Oncol, 2019, 14(1): 150. DOI: 10.1186/s13014-019-1344-7
[24] Bourton EC, Plowman PN, Zahir SA, et al. Multispectral imaging flow cytometry reveals distinct frequencies of γ-H2AX foci induction in DNA double strand break repair defective human cell lines[J]. Cytometry Part A, 2012, 81A(2): 130-137. DOI: 10.1002/cyto.a.21171
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