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| Role of complement in radiation-induced lung injury |
| GENG Shuang1, LI Qian1,2, XI Tingting1, WANG Meiyu1,3, GUO Haoxin1,2, WANG Zhixin1, BI Xiaoguang1, YAN Chengming1, YANG Zhihua1, WANG Yilong1, ZHU Maoxiang1,2,3 |
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing Key Laboratory for Radiobiology, Beijing 100850 China;
2. Nanhua University, Hengyang 421001 China;
3. College of Life Sciences, Hebei University, Baoding 071002 China |
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Abstract Objective To investigate the role of complement in radiation-induced lung injury in mice after chest irradiation with 60Co γ-rays at a single dose of 20 Gy.Methods C57BL/6 mice underwent chest irradiation with 60Co γ-rays at a single dose of 20 Gy, followed by observation for the inflammatory reaction of the lung tissue in the early stage (within 15 d) and pulmonary fibrosis in the later stage (30 and 180 d). Enzyme-linked immunosorbent assay was used to measure the levels of C2, C3a, C4, and C5b-9 in the lung tissues at 1, 3, 7, 15, 30, and 180 d after irradiation. The expression of complement mRNA in BEAS-2B cells after irradiation was determined using RT-PCR.Results Radiation-induced lung injury in micepresented as inflammatory response in the early stage and fibrosis in the late stage. Complement C2, C4, and C5b-9 complexes were increased in the early period (3 or 7 d) after irradiation (P < 0.05), which might be associated with the inflammatory response induced by irradiation. During 3 to 180 d, complement C3a was significantly higher in the irradiated mice than in the control mice, suggesting a close relationship between C3a and radiation-induced lung injury. The irradiated cells showed increased mRNA expression of C2 and C3, with no changes in the mRNA levels of C4 and C5. Conclusion Different complement proteins have varying responses to radiation-induced lung injury, among which C3a is closely related to radiation-induced lung injury, suggesting that regulating C3a and its receptors may be a new way to prevent and treat radiation-induced lung injury.
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Received: 12 April 2022
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