NHEJ通路相关基因在电离辐射所致脑损伤中的表达情况研究

doi:10.13491/j.issn.1004-714x.2019.03.001

2019, Vol. 28

Issue (3): 223-227 DOI: 10.13491/j.issn.1004-714x.2019.03.001

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Research of expression of NHEJ pathway related genes in brain injury induced by ionizing radiation

LI Mei, LIU Mengya, WANG Xingang, HUANG Liqun, SUN Ge, YIN Jingjing, QIN Xiujun

Department of Radiological and Evironmental Medicine, China Institute for Radiation Protection, Taiyuan 030006 China

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Abstract Objective To study the expression of the genes associated with the NHEJ pathway in brain injury induced by ionizing radiation on the head of rats at different doses. Methods Using medical electron accelerator to irradiate on SD rats at doses of 10Gy, 20 Gy and 30 Gy, and the total RNA in animal hippocampus tissue was extracted 30 days after exposure. The RNA of 30Gy irradiation group was extracted in 6 h, 24 h, 72 h, 7 d and 30 d after exposure. The expression of XRCC4, XRCC5 and XRCC6 genes in the NHEJ pathway wasstudied using real-time fluorescence quantitative PCR. Results The expression of genes XRCC4, XRCC5 and XRCC6 associated with DNA repair in irradiated rat hippocampus wasup-regulated. The expression levels of the genes XRCC4, XRCC5 and XRCC6 in the 20 Gy group were higher than those in 10 Gy and 30 Gy groups; The expression of XRCC4 reached the highest level 7 days after exposure, while the expression of XRCC5 and XRCC6 genes reached the highest level 6h after exposure, and the expression levels of XRCC4, XRCC5 and XRCC6 genes reached the lowest level 30 days after exposure. Conclusion The expression of genes XRCC4, XRCC5 and XRCC6 associated with DNA damage repair in the NHEJ pathway at different dose points and at different timepoints after exposure to brain injury caused by radiation was found to be raised. It indicated that the body repaired DNA double strand through NHEJ pathway at 6 h, 24 h, 72 h, 7 days and 30 days after 10, 20, 30 Gy irradiation and the most powerful repair ability was performed at 6 h and 7 days after exposure.

Key words： Radiation XRCC4 Gene XRCC5 Gene XRCC6 Gene DNA Double Strand Fracture Repair Real-Time Fluorescence Quantitative PCR

Received: 11 December 2018

PACS:

Q691.5

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	LI Mei
	LIU Mengya
	WANG Xingang
	HUANG Liqun
	SUN Ge
	YIN Jingjing
	QIN Xiujun

Cite this article:

LI Mei,LIU Mengya,WANG Xingang, et al. Research of expression of NHEJ pathway related genes in brain injury induced by ionizing radiation[J]. , 2019, 28(3): 223-227.

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http://www.zgfsws.com/EN/10.13491/j.issn.1004-714x.2019.03.001 OR http://www.zgfsws.com/EN/Y2019/V28/I3/223

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