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| Study on High Concentrations of Silica Dust Exposure Process on Rats in Oxidative Stress |
| ZHAO Jian-lei1,2,3, WANG Rui1, ZHANG Hai-dong1, WANG Hui1, ZHANG Hai-peng1, YU Yi-guang1 |
1. Shandong Academy of Medical Sciences, Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062 China;
2. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences;
3. Puji Town Center Hospital of Jiaozhou City |
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Abstract Objective To investigate the existence of oxidative stress in rats with high concentrations of silica dust exposure process. Methods Elections of 40 SD rats were randomly divided into four groups, namely high dose of dust exposure group (1000 mg/m3), middle dose of dust exposure group (500 mg/m3), low dose of dust exposure group (100 mg/m3) and the control group, Dynamic stained dust 2h every day. The rats were sacrificed after staining dust 49d, serum of superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC) activity,malondialdehyde (MDA) andreduced glutathione (GSH) levels were observed. Meanwhile,lung tissue pathological changes was observed by light microscopy morphology. Results The long, high concentrations of silica dust exposure reduced SOD, TAC, T-AOC activity and GSH contens in serum,while increasing MDA content, compared with the control group, there was a significant statistical difference (P < 0.05). Pathological examination showed inflammation lesions. Conclusion Unbalance between oxidation system and antioxidation system in vivo possible exist in high concentration silica exposure process. Oxidative stress may be one of the early symptoms of silica dust exposure.
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Received: 17 June 2014
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