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| Radioimmunoimaging of Anti-CD80 Monoclonal Antibody Labeled with 131I in Nude Mice Bearing Human B-cell lymphoma |
| ZHAI Shi-jun1, QIU Yu-hua2, WAN Wei-xing1, YU Chun-jing1, CHEN Yong-jing2, WU Yu-yu3, QI Xiao-wei3, XU Qiao-ling1 |
1. Department of Nuclear Medicine, Affiliated Hospital of Jiangnan University, The Fourth People's Hospital of Wuxi, Wuxi 214062 China;
2. Department of Medicine, Soochow University;
3. Pathological Department, Affiliated Hospital of Jiangnan University |
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Abstract Objective To explore the radioimmunoimaging of anti-CD80 monoclonal antibody labeled with 131I in nude mice bearing human lymphoma. Methods The monoclonal antibody 4E5 against human CD80 was radiolabeled with 131I using the Iodogen method at room temperature. The immune activity and stability of 131I-4E5 were analyzed. The plannar imaging of nude mice bearing human B-cell lymphoma was performed by SPECT at 24 h, 48h and 72 h after injection, and the radioactivity ratio of tumor to non-tumor tissue (T/NT) was assayed using the ROI technique. The injected dose/gramtissue percentages (% ID/g) of different organs, including tumor, blood, et al were measured by calibrator and calculated. Results The labeling rate of 131I-4E5 was (78.3 ±2.4)%, and the radiochemical purity was (95.7 ±1.8)%. The madimun specific binding efficiency of 131I-4E5 with Raji cells was (36.1 ±2.6)%. The radiochemical purity of 131I-4E5 mixing with serm was over 90% after three days. The radioimmunoimaging showed that 131I-4E5 was selectively accumulated in tumor tissue. Tumors could be clearly visualized in SPECT planar images, and the T/NT reached their peak value of 3.2 in the tumor at 72 h after injection of 131I-4E5. Conclusion 131I-4E5 antibody can target the tumor tissue in nude mice bearing human lymphoma, and provide good radioimmunoimages, which lay a foundation for further study on the radioimmunotherapy of B-cell lymphoma.
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Received: 25 May 2014
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