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| Stimulatory effect of low dose radionuclide on DNA synthesis and UDS in splenic lymphocytes |
| ZHU Shoupeng, YANG Zhanshan |
| Institute of Radiological Medicine, Suzhou Medical College, Suzhou 215007 |
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Abstract AIM To study the stimulatory effect on DNA synthesis and unscheduled DNA synthesis (UDS) in splenic lymphocytes induced by low dose enriched uranium 235U.METHODS By using 3H-TdR incorporation assay technique, the DNA replicative synthesis of splenic lymphocytes stimulated by PHA and LPS was observed.By using DNA synthesis inhibitor such as hydroxyurea, the UV-induced unscheduled DNA synthesis in splenic lymphocytes occurred.RESULTS When the injected low dose enriched uranium 235U was 0.1 μg/kg body weight, the transformation capacity was elevated for splenic T lymphocytes, simultaneously the stimulative index increased.While the UDS of splenic lymphocytes induced by ultraviolate revealed a statistically significant increase by low dose of enriched uranium 235U at the range of 0.1~20μg/kg body weight.CONCLUSION A stimulatory action of low dose enriched uranium 235U on DNA replicative synthesis as well as on UV-induced UDS in splenic lymphocytes was detected.
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Received: 19 May 1999
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