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| Effects of MiRNA-22 Expression on the Radiosensitivity of Esophageal Squamous Cell Carcinoma |
| QU Yuan1, WANG Xiao-chun2, CHE Yi-qun3 |
1. Department of Radiation Oncology, Cancer Hospital(Institute), Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100021 China;
2. Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences;
3. Department of Clinical Laboratory, Cancer Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences |
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Abstract Objective To study the effects of miRNA-22 expression on radiosensitivity of esophageal squamous cell carcinoma (ESCC). Methods Stable tranfected cell models with different miRNA-22 expression were builted. The changes of proliferation acitivity were measured by MTT and colony formation assay. Results The results of MTT and colony formation assay demonstrated that increased expression of miRNA-22 significantly inhibited the proliferation acitivity (P < 0.01) and sensitized ESCC cells to γ-ray radiation. Contrarily, decreased expression of miRNA-22 significantly promoted the proliferation acitivity (P < 0.01) and depressed radiosensitivity of ESCC cells. Conclusion These results demonstrated that the exprssion of miRNA-22 was associated with radiosensitivity of ESCC. Increased expression of miRNA-22 promoted the radiosensitivity of ESCC.
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Received: 19 March 2014
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