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| The Effect of Low-dose Neutron Irradiation on Extracellular Matrix |
| CHEN Tie-he, LU Yong-jie, CHAI Ming-sheng, et al |
| Naval Medical Research Institute, Shanghai 200431 China |
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Abstract Projective To study the effect of neutron irradiation on extracellular matrix.Methods 120 male wistar rats were divided into four groups at random, and then exposed to neutron of 252Cf-source at the doses of 0, 0.29, 0.62 and 1.20 Gy, respectively.After the exposure of 3 days, 1 month and 2 months, the rats were sacrificed and lung tissue specimens stored at -30℃.Hyaluronan, laminin, type Ⅲ procollagen and type Ⅳ collagen in the lung tissue were detected by the method of radioimmunoassay.Results The differences of the levels of hyaluronan in lung tissue among the groups were unsignificant.The levels of laminin in 0.29, 0.62 and 1.20 Gy groups after the 3-day exposure were remarkably different to those of the control group, and unable to recover completely even 2 months after the exposure.The levels of type Ⅳ collagen higher three irradiated groups were all higher, but not significantly.The levels of type Ⅲ procollagen in the early stage after exposure higher, and later they lowered.Conclusion The levels of some components of extracellular matrix in the lung tissue of rat can be changed by low-dose of neutron irradiation, but their variational modes and degrees depend on the dose of neutron irradiation and the length of period after exposure.
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Received: 18 February 2003
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