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| Ultrasound affects Apoptosis of living fetal Rat' Cardiomyocytes |
| Chen Guang-yang1, Xia Guo-yuan2 |
| Wenzhou medical college, Wenzhou 325035 China |
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Abstract Objective To study the effect of diagnostic ultrasound to living rat's embryonic cardiomyocytes on the expression of Bcl-2 and Bax protein and it's apoptosis.Methods To divided the mice which had been pregnant for 14 days into six groups:the 10 min irradiation with 2.5MHz ultrasound the 10 min irradiation with 7MHz ultrasound, the control group of 10 min, the 40 min irradiation with 2.5MHz ultrasound, the 40 min irradiation with 7MHz ultrasound and the control group of 40 min (7MHz probe:Isppa=227W/cm2;Ispta=385mW/cm2;2.5MHz probe:Isppa=94.5W/cm2, Ispta=15.6mW/cm2).Immunohistochemistry test was used to detect Bax and Bcl-2 protein's expression on fetal cardiomyocytes and TUNEL method was used to detect apoptosis.At last, TEM observation was used to observe the morphology of apoptotic cells.Results According to statistics, there had no significant difference in different embryos of the same sample (P> 0.05).Bcl-2 was weakly positive (+) and Bax was weakly positive (+) in 40 min irradiation with 7MHz ultrasound group;Bcl-2 was weakly positive (+) and Bax was negative (-) in 10 min irradiation with 7MHz ultrasound group;Bcl-2 was weakly positive (+) and Bax was negative (-) in 10 min irradiation with 2.5MHz ultrasound group;Bcl-2 was weakly positive (+) and Bax was negative (-) in 40 min irradiation with 2.5MHz ultrasound group;Two control groups were both negative (-) in Bcl-2 and Bax.TUNEL test and TEM demonstrated that 40 min irradiation with 7MHz ultrasound group was more significant than other groups in apoptosis (P<0.05).The rest of the experimental groups had no significant difference than the control groups (P<0.05).Conclusion High intensity diagnostic ultrasound can lead to apoptosis of fetal cardiomyocyte by long irradiation;Bcl-2 and Bax regulated play a regulating role in the process;Small dose of ultrasound can promote the expression of Bcl-2 protein.
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Received: 12 March 2007
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