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| Study on GVHD of mixed Bone Marrow Transplantation in Experimental Animal Models |
| BI Ke-hong1, REN Hai-quan2, ZHU Chuan-sheng1, et al |
| Shandong Qianfoshan Hospital, Jinan 250014 China |
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Abstract Objective To observe GVHD reaction in mixed BMT.Methods BALB/C mice with acute radiation disease were used as recipient mice models, C57BL/6 mice were taken as donor mice.C57BL/6 mice bone marrow and spleen cells mixed with certain percent of syngeneic spleen cells were underwent mixed BMT to observe their death rate, hematopoietic recovery of WBC lineage, spleen node count, mixed lymphocyte reaction in vivo, chimerism and pathological changes.Results GVHD was relatively strong in non-mixed BMT groups, the group mixed by 3:1 BMT after activation could not alleviate GVHD, whereas the mixed by 1:1 BMT group or by 2:1 group with high percentage of syngeneic spleen cells could lessen the extent of GVHD, as manifested by the quick recovery of peripheral blood WBC count and bone marrow GM-CFU formation, by the alleviation of mixed lymphocyte reaction in vivo as well as by the increase of chimeric rate.Conclusion Mixed BMT could lower the GVHD reaction, which was related to the ratio of syngeneic to allogeneic spleen cells.
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Received: 23 October 2006
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